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A recurrent in-frame deletion in CAPN3, c.643_663del (p.Ser215_Gly221del), has been identified in three unrelated autosomal dominant limb-girdle muscular dystrophy families, with late-onset proximal weakness accompanied by myalgia, back pain, and hyperlordosis in the fourth to fifth decades ([PMID:28881388]). The variant segregates with disease in each pedigree, and no pathogenic mutations were found in other known myopathy genes. Muscle Western blot analysis demonstrated reduced calpain-3 expression in all affected individuals, supporting a haploinsufficiency mechanism ([PMID:28881388]).
Key Take-home: Screening for CAPN3 c.643_663del (p.Ser215_Gly221del) enables molecular confirmation of the autosomal dominant calpainopathy phenotype and informs clinical management.
Gene–Disease AssociationLimited3 probands in independent AD families with segregation and initial functional data ([PMID:28881388]) Genetic EvidenceLimitedRecurrent c.643_663del in 3 unrelated families with co-segregation ([PMID:28881388]) Functional EvidenceLimitedReduced calpain-3 expression on muscle Western blot supports haploinsufficiency ([PMID:28881388]) |