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GBA1 – Perinatal Lethal Gaucher Disease

GBA1-related perinatal lethal Gaucher disease is an autosomal recessive condition characterized by near-complete glucocerebrosidase deficiency leading to nonimmune hydrops fetalis, ichthyosis, hepatosplenomegaly and rapid neonatal demise. The genetic basis involves biallelic loss-of-function variants in GBA1.

Clinical Validity and Genetic Evidence

Autosomal recessive inheritance is supported by carrier status in unaffected parents in all reported cases. Three unrelated probands have been documented ([PMID:20924719]; [PMID:31192173]; [PMID:36720536]), each presenting with nonimmune hydrops fetalis, generalized ichthyosis, hepatosplenomegaly and early death. Parental segregation confirms trans inheritance of two pathogenic alleles in each family.

Biallelic deleterious variants include splicing mutations c.1505+5G>C in exon 10 and c.308-1G>A in intron 3 observed in two fetuses with hydrops and cerebellar hypoplasia ([PMID:36720536]). No common founder alleles have been described specifically for the perinatal lethal form. Variant spectrum across all Gaucher phenotypes shows frequent nonsense, frameshift, splice-site and severe missense mutations leading to null or near-null enzyme activity.

Functional Evidence

Mechanistically, perinatal lethal Gaucher disease arises from near-complete loss of glucocerebrosidase activity, resulting in massive glucosylceramide accumulation. In vitro expression studies of severe GBA1 alleles demonstrate <1% residual activity and impaired lysosomal trafficking, while enzymatic assays in patient cells show absent activity ([PMID:31192173]). Animal and cellular models of GBA1 knockout recapitulate hydrops, skin barrier defects and neuronal apoptosis.

Integration and Conclusion

The convergence of multiple unrelated perinatal lethal cases with biallelic severe GBA1 variants, consistent autosomal recessive segregation, and extensive functional data demonstrating loss-of-function supports a Moderate clinical validity for GBA1–perinatal lethal Gaucher disease. Additional evidence from broader Gaucher disease cohorts exceeds the ClinGen scoring framework but reinforces the pathogenic mechanism.

Key Take-home: Biallelic severe GBA1 loss-of-function variants cause perinatal lethal Gaucher disease, warranting early genetic testing for rapid diagnosis and informed reproductive counseling.

References

  • Indian journal of pediatrics • 2011 • Perinatal lethal Gaucher disease. PMID:20924719
  • Frontiers in pediatrics • 2019 • A Neonatal Case With Perinatal Lethal Gaucher Disease Associated With Missense G234E and H413P Heterozygous Mutations. PMID:31192173
  • Taiwanese journal of obstetrics & gynecology • 2023 • Perinatal lethal Gaucher disease due to compound heterozygosity of the splicing mutations in GBA gene. PMID:36720536

Evidence Based Scoring (AI generated)

Gene–Disease Association

Moderate

Three unrelated perinatal lethal cases with biallelic GBA1 variants and concordant loss-of-function data

Genetic Evidence

Moderate

3 probands with AR inheritance and trans parental segregation

Functional Evidence

Moderate

In vitro and in vivo models show <1% activity and ER retention recapitulating severe phenotype