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LZTFL1 – Ciliopathy

Leucine zipper transcription factor-like 1 (LZTFL1) encodes a ciliary protein implicated in Bardet-Biedl syndrome and related ciliopathies (ciliopathy). LZTFL1 localizes to the primary cilium and regulates protein trafficking via adaptor protein complexes 1 and 2, critical for photoreceptor and renal epithelial function.

Compound heterozygous LZTFL1 variants have been reported in 2 unrelated families (4 affected individuals) presenting with rod–cone dystrophy, cognitive impairment, renal dysfunction and mesoaxial polydactyly—an uncommon digit anomaly in ciliopathies (PMID:23692385). Affected twins harbored c.260T>C (p.Leu87Pro) and c.778G>T (p.Arg260Ter) in trans, with marked reduction of LZTFL1 transcript and protein in patient fibroblasts.

Additional case series confirm a spectrum of biallelic LZTFL1 mutations—including missense, nonsense, frameshift and splice site changes—across BBS-like ciliopathy phenotypes. Recurrent mesoaxial polydactyly in all reported families suggests a diagnostic hallmark prompting early LZTFL1 sequencing.

Functional studies in Lztfl1 knockout mice demonstrate low birth weight, adult-onset obesity, shortened photoreceptor outer segments, mislocalized rhodopsin and increased apoptosis in photoreceptors, recapitulating human retinal degeneration (PMID:27312011). In vitro, LZTFL1 directly binds AP-1 and AP-2 to regulate transferrin receptor 1 trafficking; loss of LZTFL1 reduces TfR1 internalization without affecting other receptor pathways (PMID:31895934).

No conflicting reports have been published to date. The concordance of biallelic LZTFL1 variants with classical BBS features, consistent segregation and robust in vivo and in vitro functional data support a disease mechanism of loss-of-function leading to ciliary trafficking defects.

Key Take-home: Biallelic LZTFL1 variants cause an autosomal recessive ciliopathy subtype characterized by mesoaxial polydactyly, retinal degeneration and cognitive impairment—genetic testing for LZTFL1 should be prioritized in such presentations.

References

  • Clinical genetics • 2014 • Mesoaxial polydactyly is a major feature in Bardet-Biedl syndrome patients with LZTFL1 (BBS17) mutations. PMID:23692385
  • Journal of genetics and genomics • 2016 • Depletion of BBS Protein LZTFL1 Affects Growth and Causes Retinal Degeneration in Mice. PMID:27312011
  • PloS one • 2020 • Leucine zipper transcription factor-like 1 binds adaptor protein complex-1 and 2 and participates in trafficking of transferrin receptor 1. PMID:31895934

Evidence Based Scoring (AI generated)

Gene–Disease Association

Moderate

2 unrelated families (4 probands), consistent segregation and supportive functional data

Genetic Evidence

Moderate

Compound heterozygous missense and nonsense variants in 2 families with classical ciliopathy phenotypes

Functional Evidence

Moderate

Knockout mouse recapitulates retinal degeneration and trafficking assays confirm loss-of-function